Ligand Binding Studies
Our ligand binding capabilities include small molecule, protein, nucleic acid, peptide and protein-protein interactions.
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• Ligand-observe methods (1D e.g. STD/ waterLOGSY/ CPMG)
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​These type of experiments focus on the ligand's spectral properties and have no target size limitations
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Usually involve single- dimension experiments that are fast, sensitive and require relatively small amounts of both target and compound
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Provide a yes or no answer to ligand binding, but can also provide information on the ligand-target interaction
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• Target observe methods (2D eg. HSQC/HMQC)​​​
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These type of experiments focus on the targets spectral properties and do have size limitations
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The target in question usually requires 15N-(selective or uniform) labeling
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The results can provide ligand binding site identification and target conformational change that result upon ligand binding
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2D NMR methods are required
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• Binding constant determination (Kd)
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By using various 1D and 2D methods, the solution based ligand Kd can be determined
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Interested in Ligand Binding Studies Services?